SIRT2 Knockout cell line (H9)

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SIRT2 Knockout cell line (H9) Order

Catalog#

YKO-H1123

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

SIRT2 Knockout cell line（H9）

Cell Morphology

Spherical clone

Passage ratio

1:6-1:8

Species

Human

Gene Name

SIRT2

Gene ID

22933

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

EZ-Stem™ Cell Culture Medium

Accession

CVCL_D8GE(Cellosaurus)

Target region

Knockout region: Exon 4 (based on transcript SIRT2-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:H9			STR Info (Cell bank) Cell Line:WAe009-A-5
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	9	9		9	9
D7S820	9	11		9	11
D16S539	12	13		12	13
VWA	17	17		17	17
TH01	9.3	9.3		9.3	9.3
AMEL	X	X		X	X
TPOX	10	11		10	11
CSF1PO	11	11		11	11
D12S391	15	19
FGA	26	28
D2S1338	18	24
D21S11	30	30
D18S51	13	13
D8S1179	8	14
D3S1358	13	16
D6S1043	12	19
PENTAE	11	14
D19S433	12	15
PENTAD	9	13
D1S1656	12	13

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SIRT2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the H9 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SIRT2 gene knockout SIRT2 Knockout cell line（H9） will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

SIRT2 Knockout cell line (H9)

Catalog#

YKO-H1123

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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SIRT2 Knockout cell line (H9)

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